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Updated: Aug 12, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
Analysis of one-component saturable systems such as ligand binding, enzyme kinetic, uptake, and transport data
Abstract:
Statistical methods for analyzing enzyme kinetic or drug binding assays involving simple first-order kinetics have been reviewed. The Eadie-Hofstee format combines many attractive features. Specifically, this plot is as efficient as any of the alternatives, shows no tendency to produce grossly aberrant values, does not require explicit weighting, does not require an iterative fitting process, gives the parameters of interest and their errors of estimate directly, and is unbiased (after correction). A correction for the bias inherent in the Eadie-Hofstee format has been developed. A numerical example and computer program are given in appendices.
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