Evaluation of lncRNA Expression Pattern and Potential Role in Heart Failure Pathology

Area of Science:

• Cardiovascular Biology
• Molecular Genetics
• Biomarker Discovery

Background:

• Heart failure (HF) remains a significant cause of morbidity and mortality globally.
• Current diagnostic and therapeutic strategies for HF require further advancement.
• The role of long noncoding RNAs (lncRNAs) in HF pathogenesis is not fully understood.

Purpose of the Study:

• To identify novel long noncoding RNAs (lncRNAs) as potential diagnostic and therapeutic targets for heart failure (HF).
• To explore the underlying molecular mechanisms associated with differentially expressed lncRNAs in HF patients.

Main Methods:

• RNA sequencing was performed on peripheral blood from healthy controls and HF patients.
• lncRNA expression was validated using RT-qPCR.
• Bioinformatic analyses, including GO, KEGG pathway, and lncRNA-mRNA coexpression network analysis, were conducted.
• Receiver Operating Characteristic (ROC) analysis was used to assess the diagnostic value of lncRNAs.

Main Results:

• A total of 207 mRNAs and 422 lncRNAs were identified as differentially expressed.
• Key biological pathways implicated in HF include immune response, cell membrane regulation, and transcriptional regulation.
• Four specific lncRNAs (CHST11, MIR29B2CHG, CR381653.1, and FP236383.2) demonstrated potential diagnostic value for HF.

Conclusions:

• Novel lncRNAs have been identified as potential biomarkers for HF diagnosis.
• These findings offer new insights into HF mechanisms and potential therapeutic targets.
• The identified lncRNAs may contribute to improved diagnostic accuracy and treatment strategies for heart failure.