CNT-FET for sensitive hydrogen peroxide biosensing via immobilized Cytochrome c

H₂O₂ is an effective substance in the body which contributes to gene expression, insulin metabolism and determining cell shapes. However, a high concentration of H₂O₂ is harmful to the body and can cause various diseases such as colitis wounds, sepsis disease, lymphocyte proliferation and macrophage apoptosis in systemic lupus erythematosus. In this study, a Cyt c/cMWCNTs/FET was designed to real-time detect H₂O₂ via immobilized Cyt c on the cMWCNTs/FET surface. The performance of the Cyt c/cMWCNTs/FET biosensor was studied under various parameters such as cMWCNTs and Cyt c concentrations, as well as different pH values. When H₂O₂ was added to the reaction chamber of the Cyt c/cMWCNTs/FET, the output current of the Bio-FET was reduced, which was attributed to H₂O₂ detection. The linear response range of this Cyt c/cMWCNT/FET was 10.0 fM to 1.0 nM. The limit of detection and response time of this platform were determined to be 9.13 fM and around 1.0 s, respectively. Also, the operation of the Cyt c/cMWCNTs/FET in the presence of glucose, leucine, tyrosine and ascorbic acid as interfering substances was selective towards H₂O₂.