Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.1K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.1K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

247
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
247
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

401
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
401
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

740
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
740
Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

744
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
744

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Controlled Amine-Borane Dehydropolymerization Enabled by Mechanistic Insight Using the Ir(<sup>t</sup>Bu-POCOP)H<sub>2</sub> Catalyst.

Journal of the American Chemical Society·2026
Same author

Evidence for Transient Ylide Intermediates of the Type <sup>t</sup>Bu<sub>2</sub>P(H)=CHZ That Are Prototropic Tautomers of <sup>t</sup>Bu<sub>2</sub>PCH<sub>2</sub>Z.

Inorganic chemistry·2026
Same author

Clinical care patterns in the 10-years prior to primary total hip replacement: a matched population-based case-control analysis in England.

BMC medicine·2026
Same author

Aryl Boroxines via Rotary Evaporation.

Organic letters·2026
Same author

Correction: A consolidated framework for implementation research (CFIR) informed exploration of a primary care intervention to support deprescribing for problematic polypharmacy in older adults living with frailty (DEPPLOY) in England: a qualitative study.

International journal of clinical pharmacy·2026
Same author

A Stopped-Flow Instrument for Millisecond Timescale Reaction Monitoring on a Standard NMR Spectrometer.

Magnetic resonance in chemistry : MRC·2026

Related Experiment Video

Updated: Jul 22, 2025

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

6.5K

SHARPER-DOSY: Sensitivity enhanced diffusion-ordered NMR spectroscopy.

George Peat1, Patrick J Boaler1, Claire L Dickson1,2

  • 1EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Rd, Edinburgh, EH9 3FJ, UK.

Nature Communications
|July 21, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a new liquid-state Nuclear Magnetic Resonance (NMR) method for highly sensitive diffusion measurements. This technique enables precise size estimations of compounds using minimal material, saving significant time.

More Related Videos

Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
11:44

Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

Published on: November 12, 2016

18.0K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.0K

Related Experiment Videos

Last Updated: Jul 22, 2025

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging
06:34

In Situ Monitoring of Diffusion of Guest Molecules in Porous Media Using Electron Paramagnetic Resonance Imaging

Published on: September 2, 2016

6.5K
Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
11:44

Spin Saturation Transfer Difference NMR SSTD NMR: A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes

Published on: November 12, 2016

18.0K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.0K

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Spectroscopy

Background:

  • Nuclear Magnetic Resonance (NMR) sensitivity has improved with advanced experimental designs.
  • Measuring diffusion coefficients is crucial for estimating molecular size.

Purpose of the Study:

  • To develop a liquid-state NMR method for enhanced sensitivity in diffusion coefficient measurements.
  • To enable molecular size estimation using significantly reduced sample quantities.

Main Methods:

  • Utilizing narrow, intense singlets invariant to magnetic field inhomogeneities.
  • Employing short spin-echo intervals (<0.5 ms) with non-selective pulses to suppress chemical shift and J-coupling evolution.
  • Acquiring signals within these optimized intervals.

Main Results:

  • Achieved a 10-100 fold sensitivity enhancement in diffusion measurements.
  • Resulted in a 100-10000 fold reduction in measurement time.
  • Enabled diffusion coefficient measurement of medium-sized organic molecules in minutes with only hundreds of nanograms of material.

Conclusions:

  • The developed NMR methodology offers a substantial increase in sensitivity and efficiency for diffusion measurements.
  • This technique significantly reduces the required sample amount, making it ideal for analyzing precious or limited materials.
  • The method facilitates rapid molecular size determination using advanced NMR instrumentation.