Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of a Selective Histone Deacetylase 6 Degrader (HDAC6 PROTAC) with a Short and Rigid Linker Demonstrating Sustained Knockdown of HDAC6 <i>In Vivo</i>.

ACS medicinal chemistry letters·2026
Same author

The spatial proteome of the Plasmodium falciparum schizont illuminates the composition and evolutionary trajectories of its organelles.

Nature communications·2026
Same author

Potential Diagnostic and Research Applications of a Recombinant Antibody Directed Against Ferrated Triacetylfusarinine C from <i>Aspergillus fumigatus</i>.

Journal of fungi (Basel, Switzerland)·2026
Same author

A divergent Plasmodium NEK4 acts as a key regulator driving the early events of meiosis.

Nature communications·2026
Same author

Dynamic subcellular proteomics identifies regulators of adipocyte insulin action.

Nature communications·2026
Same author

Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex.

Nature communications·2026

Related Experiment Video

Updated: Jul 22, 2025

Chemical Cartography Approaches to Study Trypanosomatid Infection
08:21

Chemical Cartography Approaches to Study Trypanosomatid Infection

Published on: January 21, 2022

2.4K

Mapping diversity in African trypanosomes using high resolution spatial proteomics.

Nicola M Moloney1, Konstantin Barylyuk1, Eelco Tromer2

  • 1Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK.

Nature Communications
|July 21, 2023
PubMed
Summary
This summary is machine-generated.

This study maps the spatial proteomes of African trypanosomes, revealing over 2500 proteins per cell type in distinct subcellular compartments. This provides insights into parasitic adaptation and diversity in human and animal diseases.

More Related Videos

High-throughput Gene Tagging in Trypanosoma brucei
11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

8.0K
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
10:04

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Published on: August 19, 2014

9.0K

Related Experiment Videos

Last Updated: Jul 22, 2025

Chemical Cartography Approaches to Study Trypanosomatid Infection
08:21

Chemical Cartography Approaches to Study Trypanosomatid Infection

Published on: January 21, 2022

2.4K
High-throughput Gene Tagging in Trypanosoma brucei
11:26

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

8.0K
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
10:04

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Published on: August 19, 2014

9.0K

Area of Science:

  • Parasitology
  • Proteomics
  • Molecular Biology

Background:

  • African trypanosomes are eukaryotic parasites causing significant disease in sub-Saharan Africa.
  • Species and life-cycle stages exhibit distinct host and tissue tropisms, contributing to disease diversity.

Purpose of the Study:

  • To map the spatial proteomes of two African trypanosome species, Trypanosoma brucei and Trypanosoma congolense, across two life stages.
  • To provide comprehensive spatial proteomes for four distinct cell types.
  • To analyze parasitic adaptation and the molecular basis of diversity.

Main Methods:

  • Spatial proteome mapping of Trypanosoma brucei and Trypanosoma congolense.
  • Analysis across two distinct life stages.
  • Classification of proteins to specific subcellular compartments.

Main Results:

  • Expression of approximately 5500 proteins per cell-type was detected.
  • Over 2500 proteins per cell-type were localized to specific subcellular compartments.
  • Four comprehensive spatial proteomes were generated.

Conclusions:

  • Comparative analysis revealed key parasitic adaptation routes to different biological niches.
  • The study offers insights into the molecular basis of diversity within and between trypanosome species.
  • Understanding spatial proteomes aids in developing strategies against African trypanosomiasis.