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KNTC1 and MCM2 are the molecular targets of gallbladder cancer
1Department of Gastrointestinal Surgery, Beijing Rehabilitation Hospital, Capital Medical University, Badachu Xixia, Shijingshan 100144, Beijing, China.
Background:
Gallbladder carcinoma is a malignant epithelial tumor of gallbladder with a high degree of malignancy. However, relationship between KNTC1 and MCM2 and gallbladder cancer is unclear.
Methods:
GSE139682 and GSE202479 were downloaded from gene expression omnibus (GEO). Differentially expressed genes (DEGs) were screened. Functional enrichment analysis and gene set enrichment analysis (GSEA) were performed. Protein-protein interaction (PPI) Network was constructed and analyzed. Gene expression heat map was drawn. Comparative toxicogenomics database (CTD) analysis was performed to find diseases most related to core genes. TargetScan was performed for screening miRNAs that regulated central DEGs.
Results:
230 DEGs were identified. According to GObp analysis, they were mainly concentrated in regulation of ossification, regulation of spindle microtubule and centromere attachment, cytoskeleton tissue of cortical actin. According to GOcc analysis, they are mainly concentrated in plasma membrane part, cell junction, plasma membrane region and anterior membrane. According to GOmf analysis, they are mainly enriched in protein homodimerization activity, proximal promoter sequence-specific DNA binding and sulfur compound binding. KEGG showed that target genes were mainly enriched in Hippo signal pathway, p53 signal pathway and cancer pathway. KIFC2, TUBG1, RACGAP1, CHMP4C, SFN and MYH11 were identified as core genes. Gene expression heat map showed that KNTC1, MCM2, CKAP2, RACGAP1, CCNB1 were highly expressed in gallbladder carcinoma samples. CTD analysis showed that KNTC1, MCM2, CKAP2, RACGAP1, CCNB1 were associated with head and neck squamous cell carcinoma, necrosis, inflammation and hepatomegaly.
Conclusions:
KNTC1 and MCM2 are highly expressed in gallbladder cancer. Higher expression level correlates with worse prognosis.
Insights
This study reveals that KNTC1 and MCM2 are highly expressed in gallbladder cancer, with increased levels correlating to a worse prognosis for patients. These findings highlight potential biomarkers for this malignancy.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Gallbladder carcinoma is a highly malignant epithelial tumor.
- The specific roles of KNTC1 and MCM2 in gallbladder cancer development remain unclear.
Purpose of the Study:
- To investigate the expression patterns and potential roles of KNTC1 and MCM2 in gallbladder carcinoma.
- To identify differentially expressed genes and associated pathways in gallbladder cancer.
Main Methods:
- Downloaded and analyzed gene expression data from GEO (GSE139682, GSE202479).
- Performed differential gene expression analysis, functional enrichment (GO, KEGG), and pathway analysis (GSEA).
- Constructed a protein-protein interaction network and utilized the Comparative Toxicogenomics Database (CTD).
Main Results:
- Identified 230 differentially expressed genes (DEGs) enriched in pathways like Hippo and p53 signaling.
- Highlighted KNTC1, MCM2, CKAP2, RACGAP1, and CCNB1 as highly expressed in gallbladder cancer samples.
- Found associations between these genes and conditions including head and neck squamous cell carcinoma, necrosis, inflammation, and hepatomegaly via CTD analysis.
Conclusions:
- KNTC1 and MCM2 are significantly overexpressed in gallbladder cancer.
- Elevated expression of KNTC1 and MCM2 is linked to a poorer patient prognosis, suggesting their potential as prognostic biomarkers.
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