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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
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Surface plasmon resonance-based oligonucleotide biosensor for Salmonella Typhi detection.

Sepideh Fathi1, Nazila Jalilzadeh2, Mohammad Amini2

  • 1Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab-Rashid, Tabriz, Iran; Immunology Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.

Analytical Biochemistry
|July 23, 2023
PubMed
Summary

This study presents a novel Surface Plasmon Resonance (SPR) biosensor for accurate typhoid fever diagnosis. The developed genosensor effectively detects Salmonella typhi DNA, offering a promising tool for disease management.

Keywords:
BiosensorOligonucleotideSPRSalmonella typhiSurface plasmon resonance

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Infectious Diseases

Background:

  • Typhoid fever remains a significant global health issue, especially in developing nations, due to high mortality rates.
  • Current diagnostic methods for typhoid fever face challenges with specificity and sensitivity, leading to frequent false-negative results.
  • Accurate and rapid detection of Salmonella typhi is crucial for effective typhoid fever management.

Purpose of the Study:

  • To design and develop a Surface Plasmon Resonance (SPR) based biosensor for the specific detection of Salmonella typhi.
  • To evaluate the sensitivity and specificity of the developed genosensor for typhoid fever diagnosis.
  • To assess the reusability of the biosensor for practical diagnostic applications.

Main Methods:

  • Development of a Surface Plasmon Resonance (SPR) biosensor utilizing DNA hybridization for Salmonella typhi detection.
  • Testing the biosensor with synthetic target sequences and PCR products to determine detection limits.
  • Evaluating the genosensor's ability to differentiate between complementary and single-base mismatch sequences.
  • Assessing the regeneration capabilities of the biosensor surface using NaOH solution.

Main Results:

  • The designed SPR biosensor demonstrated successful specific detection of Salmonella typhi DNA through hybridization.
  • The lowest detectable concentrations were 10 nM for synthetic target sequences and 1 nM for PCR products.
  • The genosensor accurately distinguished complementary sequences from those with a single base mismatch.
  • The biosensor surface showed effective regeneration, allowing for consecutive diagnostic cycles.

Conclusions:

  • The developed SPR-based genosensor exhibits high sensitivity and specificity for Salmonella typhi detection.
  • This biosensor presents a promising and potentially practical tool for improving typhoid fever diagnosis.
  • The ability to regenerate the sensor surface enhances its utility for repeated diagnostic use.