Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

10.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.0K
The Replisome03:01

The Replisome

33.9K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.9K
DNA Bacteriophages01:26

DNA Bacteriophages

52
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
52
Chromosome Structure02:40

Chromosome Structure

22.9K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.9K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
Replication in Prokaryotes02:35

Replication in Prokaryotes

87.6K
Overview
87.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Building Molecules by a Self-Replicator That Catalyzes Acyl Hydrazone Formation.

Angewandte Chemie (International ed. in English)·2026
Same author

Departure from randomness: Evolution of self-replicators that can self-sort through steric zipper formation.

Chem·2025
Same author

Covalent Dynamic DNA Networks to Translate Multiple Inputs into Programmable Outputs.

Journal of the American Chemical Society·2025
Same author

Competitive exclusion among self-replicating molecules curtails the tendency of chemistry to diversify.

Nature chemistry·2024
Same author

Simultaneous Formation of a Foldamer and a Self-Replicator by Out-of-Equilibrium Dynamic Covalent Chemistry.

Journal of the American Chemical Society·2024
Same author

Light-Mediated Interconversion between a Foldamer and a Self-Replicator.

Journal of the American Chemical Society·2024
Same journal

Switching Site Selectivity in Alkoxyamine Hydration: From Lone-Pair Direction to Solvent Network Dominance.

Journal of the American Chemical Society·2026
Same journal

A Topotactic Leap: 2D Layers to 3D Large-Pore Zeolite.

Journal of the American Chemical Society·2026
Same journal

Enhanced Hydrogen Evolution over Single-Atom Catalysts via Electrostatic Polarization in Contact-electro-catalysis.

Journal of the American Chemical Society·2026
Same journal

Tumor Acidity-Activatable Ionizable Lipid Nanoparticles for Selective Oncolytic Therapy.

Journal of the American Chemical Society·2026
Same journal

Alternating Magnetic Field Promotes Ammonia Cracking by Disrupting the Sabatier Limitation of Ruthenium Catalytic Species.

Journal of the American Chemical Society·2026
Same journal

Bulk Ferromagnetic Icosahedral Quasicrystals without Rapid Quenching.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jul 22, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

7.3K

Enantioselective Self-Replicators.

Shuo Yang1,2,3, Yannick Geiger2, Marc Geerts2

  • 1State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Journal of the American Chemical Society
|July 24, 2023
PubMed
Summary
This summary is machine-generated.

Chirality influences self-replicating molecules, crucial for life's origin. Researchers developed enantioselective self-replicators that sort chiral precursors, advancing the study of life's emergence.

More Related Videos

Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

3.4K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.8K

Related Experiment Videos

Last Updated: Jul 22, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

7.3K
Chemical Triphosphorylation of Oligonucleotides
13:19

Chemical Triphosphorylation of Oligonucleotides

Published on: June 2, 2022

3.4K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.8K

Area of Science:

  • Chemistry
  • Origin of Life Research
  • Supramolecular Chemistry

Background:

  • Self-replicating molecules are key to understanding life's origins.
  • Chirality is fundamental to life, yet its role in early self-replication is understudied.

Purpose of the Study:

  • To investigate the impact of chirality on self-replicating molecules.
  • To develop enantioselective self-replicators for studying life's emergence.

Main Methods:

  • Synthesized self-assembled self-replicators.
  • Assessed enantioselectivity and precursor incorporation.
  • Studied structural influences on enantioselectivity.

Main Results:

  • Developed novel self-assembled self-replicators exhibiting spontaneous enantioselectivity.
  • Achieved efficient sorting of chiral precursors into enantiopure replicators.
  • Identified structural features (ring size) governing enantioselectivity.

Conclusions:

  • Enantioselective self-replicators can emerge and grow from enantiopure material.
  • These replicators can sort racemic precursors based on chirality.
  • Structural constraints play a critical role in the enantioselectivity of self-replicators, advancing de novo synthesis of life.