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Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

537
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Electrophoresis: Overview01:20

Electrophoresis: Overview

2.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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Exploring Biopharmaceutical Analysis with Compact Capillary Liquid Chromatography Instrumentation.

Benjamin P Libert1, Samuel W Foster1, Elisabeth P Gates2

  • 1Rowan University.

LC GC Europe
|July 24, 2023
PubMed
Summary
This summary is machine-generated.

Miniaturized liquid chromatography (LC) systems offer portable analysis for pharmaceutical manufacturing. A portable LC instrument coupled with mass spectrometry (MS) was used for monoclonal antibody (mAb) characterization.

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Area of Science:

  • Analytical Chemistry
  • Biopharmaceutical Analysis
  • Process Analytical Technology (PAT)

Background:

  • Miniaturization of analytical instrumentation is a growing trend.
  • Portable liquid chromatography (LC) systems enable on-site analysis, reducing sample transport.
  • In-situ monitoring is crucial for pharmaceutical and biopharmaceutical manufacturing.

Purpose of the Study:

  • To describe a portable, miniaturized LC instrument.
  • To demonstrate its application in characterizing a biopharmaceutical monoclonal antibody (mAb).
  • To highlight the utility of portable LC-MS in manufacturing environments.

Main Methods:

  • Development and implementation of a portable, miniaturized LC system.
  • Coupling of the LC system to a mass spectrometer (MS).
  • Application of the LC-MS system for the characterization of a monoclonal antibody (mAb).

Main Results:

  • Successful characterization of a biopharmaceutical monoclonal antibody (mAb) using the portable LC-MS system.
  • Demonstration of the feasibility of on-site analysis in a manufacturing setting.
  • Validation of miniaturized LC technology for biopharmaceutical quality control.

Conclusions:

  • Portable, miniaturized LC-MS instruments are suitable for biopharmaceutical characterization.
  • These systems enhance flexibility and enable real-time process monitoring.
  • The technology supports efficient quality control directly within manufacturing environments.