Related Experiment Video
Updated: Jul 22, 2025
08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
12.3K
Corrigendum: Evaluation of CircRNA sequence assembly methods using long reads
Jingjing Zhang1,2, Md Tofazzal Hossain1,2, Weiguo Liu3
1University of Chinese Academy of Sciences, Beijing, China.
Frontiers in Genetics
|July 24, 2023
Abstract:
[This corrects the article DOI: 10.3389/fgene.2022.816825.].
Related Concept Videos
03:21RNA-seq
10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
03:36Genome Annotation and Assembly
18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
02:35RACE - Rapid Amplification of cDNA Ends
6.4K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.4K