Atomization-Induced High Intrinsic Activity of a Biocompatible MgAl-LDH Supported Ru Single-Atom Nanozyme for...

Developing efficient nanozymes to mimic natural enzymes for scavenging reactive radicals remains a significant challenge owing to the insufficient activity of conventional nanozymes. Herein, we report a novel Ru single-atom nanozyme (SAE), featuring atomically dispersed Ru atoms on a biocompatible MgAl-layered double hydroxide (Ru₁ /LDH). The prepared Ru₁ /LDH SAE shows high intrinsic peroxidase (POD)-like catalytic activity, which outperforms the Ru nanoclusters (NCs) nanozyme by a factor of 20 and surpasses most SAEs. The density functional theory calculations reveal that the high intrinsic POD-like activity of Ru₁ /LDH can be attributed to a heterolytic path of H₂ O₂ dissociation on the single Ru sites, which requires lower free energy (0.43 eV) compared to the homolytic path dissociation on Ru NC (0.63 eV). In addition, the Ru₁ /LDH SAE shows excellent multiple free radicals scavenging ability, including superoxide anion radical (O₂ ⋅^- ), hydroxyl radical (⋅OH), nitric oxide radical (NO⋅) and 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH⋅). Given the advantages of Ru₁ /LDH with high enzymatic activities, biosafety, and ease to scale up, it paves the way for exploring SAEs in the practical biological immunity system.