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Updated: Jul 22, 2025

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
Published on: May 10, 2024
807
CRISPR/Cas, Multiomics, and RNA Interference in Virus Disease Management
Kotapati Kasi Viswanath1, Aflaq Hamid1, Elijah Ateka2
1Department of Plant Pathology, Washington State University, Pullman, WA, U.S.A.
Phytopathology
|July 24, 2023
Summary
New molecular tools like CRISPR and RNA interference offer precise plant virus management strategies. These advanced techniques, alongside omics data, enhance virus resistance in crops, complementing traditional methods.
Area of Science:
- Plant pathology
- Molecular biology
- Genetics
Background:
- Plant viruses cause significant global crop losses.
- Emerging viral strains and resistance necessitate novel management strategies.
- Conventional methods require complementary advanced approaches.
Purpose of the Study:
- To review recent advancements in molecular tools for plant virus disease management.
- To highlight the integration of omics data for enhancing virus resistance.
- To discuss the application of CRISPR and RNA interference technologies.
Main Methods:
- Review of CRISPR/Cas systems for genome editing.
- Analysis of transcriptomics for host-virus interactions.
- Examination of RNA interference (endogenous and exogenous dsRNA) applications.
Main Results:
- CRISPR and RNA interference enable precise targeting of viral components.
- Omics data accelerates understanding of host-virus interactions.
- These technologies offer new avenues for developing virus-resistant crops.
Conclusions:
- Advanced molecular tools like CRISPR and RNAi are crucial for effective plant virus management.
- Integrating omics data with these tools can expedite breeding for virus resistance.
- Continued research is vital for developing sustainable virus control strategies.
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