Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.9K
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

4.2K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the Gold Electrode Standard: Using a Triazabutadiene-Maleimide Linker to Covalently Immobilize a Glycan-Polymer Biorecognition Element onto Carbon.

ACS applied bio materials·2026
Same author

Nanodisc-Forming Polymers for the Extraction of Membrane Proteins.

Biomacromolecules·2026
Same author

Core-block engineering enables control of ice recrystallisation inhibition in polymer nanoparticles.

Chemical science·2026
Same author

Antifreeze Glycolipid-Inspired Ice Recrystallization Inhibitors Based on End-Group Functionalized Poly(vinyl alcohol).

ACS macro letters·2026
Same author

Poly(sarcosine)-<i>block</i>-oligo(l-tryptophan) Copolymers as Noncovalent Inhibitors of Protein Aggregation.

ACS macro letters·2026
Same author

Cryopreservation of Avian Erythrocytes Using Macromolecular Cryoprotectants.

ACS polymers Au·2026

Related Experiment Video

Updated: Jul 22, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.3K

Programmable Monodisperse Glyco-Multivalency Using Self-Assembled Coordination Cages as Scaffolds.

Callum Pritchard1, Melissa Ligorio1, Garrett D Jackson1

  • 1Department of Chemistry, University of Warwick, Coventry CV47AL, U.K.

ACS Applied Materials & Interfaces
|July 24, 2023
PubMed
Summary

Researchers developed precisely controlled glycan clusters using metal coordination cages. This breakthrough offers predictable valency for enhanced lectin binding, advancing glycomaterial applications in sensing and imaging.

Keywords:
coordination cagesglycanslectinsmultivalencyself-assembly

More Related Videos

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
14:37

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

11.5K
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
11:08

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

8.8K

Related Experiment Videos

Last Updated: Jul 22, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.3K
High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
14:37

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

11.5K
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
11:08

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

8.8K

Area of Science:

  • Carbohydrate Chemistry
  • Supramolecular Chemistry
  • Materials Science

Background:

  • Multivalent glycan presentation enhances lectin binding via the cluster glycoside effect.
  • Conventional scaffolds (polymers, nanoparticles) exhibit dispersity, limiting control and reproducibility of glycan valency.
  • This inherent dispersity complicates identifying specific glycan fractions responsible for binding.

Purpose of the Study:

  • To create perfectly monodisperse glycoclusters with precisely controlled valency.
  • To overcome limitations of dispersity in traditional glycan array materials.
  • To explore the impact of controlled valency on lectin binding.

Main Methods:

  • Utilized self-assembled metal coordination cages for structurally programmable scaffolds.
  • Assembled a library of 16 monodisperse glycoclusters with valencies ranging from 2 to 24.
  • Introduced mono- and trisaccharides, including biologically relevant sialic acids, into the clusters.

Main Results:

  • Demonstrated a synthetic route tolerant of biologically relevant glycans.
  • Observed increased binding to various lectins as glycan valency increased.
  • Achieved perfectly monodisperse glycan arrays with predictable valency.

Conclusions:

  • Metal coordination cages provide precise control over 3-D glycan array valency, surpassing current glycomaterials.
  • This strategy offers enhanced reproducibility and predictability for glycan-based materials.
  • Potential applications span sensing, imaging, and fundamental biological research.