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In vitro propagation of infectious hypodermal hematopoietic necrosis virus (Penaeus stylirostris penstyldensovirus)

Dominic Vithayathil Diya Dominic1, Thavarool Puthiyedathu Sajeevan1, Ahna Ameer1

  • 1National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.

Archives of Virology
|July 24, 2023
PubMed

Insights

Researchers successfully propagated Infectious Hypodermal Hematopoietic Necrosis Virus (IHHNV) in a novel shrimp-insect cell line. This breakthrough enables further study of IHHNV infection and the development of antiviral strategies for aquaculture.

Area of Science:

  • Aquaculture
  • Virology
  • Cell Biology

Background:

  • Infectious Hypodermal Hematopoietic Necrosis Virus (IHHNV) poses a significant threat to shrimp aquaculture.
  • Understanding IHHNV replication and pathogenesis is crucial for developing effective control measures.

Purpose of the Study:

  • To establish a reliable in vitro system for studying IHHNV.
  • To investigate the replication of IHHNV in a hybrid shrimp-insect cell line.

Main Methods:

  • Isolation and propagation of IHHNV in the PmLyO-Sf9 hybrid cell line.
  • Observation of cytopathic effects using light and transmission electron microscopy.
  • Purification and characterization of the virus.

Main Results:

  • Cytopathic changes, including cell death and syncytium formation, were observed post-inoculation.
  • Viral gene expression and virion presence confirmed IHHNV replication in the cell line.
  • Purified IHHNV demonstrated infectivity in vitro and in vivo.

Conclusions:

  • The PmLyO-Sf9 cell line serves as a viable model for IHHNV research.
  • This system facilitates studies on IHHNV molecular mechanisms, pathogenesis, and replication.
  • The findings support the development of novel antiviral strategies for shrimp aquaculture.

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