Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

52.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.4K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

64
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
64
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MicroRNA expression signatures associated with metastatic progression in papillary thyroid carcinoma.

Archives of endocrinology and metabolism·2026
Same author

MicroRNA signatures associated with radioiodine refractoriness and tumor dedifferentiation in metastatic papillary thyroid carcinoma.

Endocrine·2026
Same author

Differences in the aggressiveness of familial versus sporadic non-medullary thyroid cancer: An unresolved controversy.

Archives of endocrinology and metabolism·2026
Same author

Editorial: New molecular pathways in thyroid biology: role of coding and noncoding genes in thyroid pathophysiology, volume II.

Frontiers in endocrinology·2025
Same author

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer.

FEBS letters·2025
Same author

Tertiary lymphoid structures in thyroid cancer.

Archives of endocrinology and metabolism·2025

Related Experiment Video

Updated: Jul 22, 2025

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

1.0K

Using CRISPR/Cas9 to Edit a Thyroid Cancer Cell Line.

Cesar Seigi Fuziwara1, Edna Teruko Kimura2

  • 1Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.

Advances in Experimental Medicine and Biology
|July 24, 2023
PubMed
Summary

Thyroid cancer, the most common endocrine cancer, involves genetic changes in MAPK signaling. Researchers are using CRISPR/Cas9 gene-editing technology to study thyroid cancer cell lines and understand its biology.

More Related Videos

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
10:21

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

13.2K
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
07:56

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Published on: July 22, 2019

11.3K

Related Experiment Videos

Last Updated: Jul 22, 2025

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

1.0K
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
10:21

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

13.2K
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
07:56

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Published on: July 22, 2019

11.3K

Area of Science:

  • Endocrinology
  • Oncology
  • Molecular Biology

Background:

  • Thyroid cancer is the most common endocrine malignancy, characterized by diverse subtypes and distinct clinical-pathological features.
  • Oncogenesis is linked to genetic alterations in MAPK signaling pathways, affecting cell proliferation and noncoding gene expression (microRNAs, long noncoding RNAs).

Purpose of the Study:

  • To explore the application of CRISPR/Cas9 technology in investigating thyroid cancer biology.
  • To review current in vitro studies utilizing CRISPR/Cas9 in thyroid cancer research.

Main Methods:

  • CRISPR/Cas9 gene-editing system.
  • In vitro studies on thyroid cancer cell lines.
  • Analysis of gene sequence modification and gene expression modulation.

Main Results:

  • CRISPR/Cas9 enables precise gene editing in thyroid cancer cells.
  • The technology facilitates the study of gene functions in thyroid cancer development.
  • Modulation of noncoding RNAs and MAPK signaling pathways can be investigated.

Conclusions:

  • CRISPR/Cas9 is a valuable tool for advancing thyroid cancer research.
  • In vitro applications of CRISPR/Cas9 are crucial for understanding thyroid cancer biology.
  • Further research using CRISPR/Cas9 may lead to novel therapeutic strategies.