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Published on: August 23, 2019
miR-214-5p increases the radiosensitivity of cervical cancer by targeting ROCK1 expression
Junqin Zhang1, Yaxing Li2, Yanan Ren1
1Department of Gynecology, Hebei General Hospital, Shijiazhuang, China.
Background:
The tolerance of cervical cancer to radiotherapy is a major factor affecting treatment outcomes. The miR-214-5p is involved in the regulation of biological processes such as tumor proliferation and metastasis.
Objectives:
The aim of the study was to explore the role of miR-214-5p and Rho-associated coiled-coil containing protein kinase 1 (ROCK1) in cervical cancer and their response to radiotherapy in cervical cancer patients.
Material And Methods:
Fifty-three cervical cancer tissue samples were collected to analyze the level of miR-214-5p in patients with different responses to radiotherapy. Cervical cancer cell lines with radiation resistance were selected to explore the role of miR-214-5p in radiosensitivity. The wound healing, transwell migration, clone formation assay, and in vivo analysis were utilized to evaluate the effect of miR-214-5p on the radiation sensitivity of cervical cancer cells.
Results:
Patients with poor radiotherapy responses demonstrated low levels of miR-214-5p. The upregulation of miR-214-5p decreased migration and invasion ability of radiotherapy-resistant cells. The bioinformatic analysis showed that ROCK1 is a candidate target gene of miR-214-5p, and this was confirmed with dual luciferase reporter assay showing that miR-214-5p directly interacts with the 3'untranslated region (3'UTR) of ROCK1. Decreased ROCK1 improved the radiosensitivity of cervical cancer in vitro and in vivo, and the overexpression of ROCK1 decreased the radiosensitivity effect of miR-214-5p in cervical cancer cells.
Conclusions:
The miR-214-5p can regulate the radiation sensitivity of cervical cancer cells by targeting the mRNA of ROCK1 and regulating its expression.
Insights
MicroRNA-214-5p (miR-214-5p) enhances cervical cancer radiosensitivity by targeting ROCK1. Low miR-214-5p levels correlate with poor radiotherapy response, suggesting its therapeutic potential.
Area of Science:
- Oncology
- Molecular Biology
- Radiotherapy Research
Background:
- Cervical cancer exhibits tolerance to radiotherapy, impacting treatment success.
- MicroRNA-214-5p (miR-214-5p) influences tumor proliferation and metastasis.
- Understanding miR-214-5p and ROCK1 roles is crucial for improving cervical cancer radiotherapy.
Purpose of the Study:
- To investigate the role of miR-214-5p and Rho-associated coiled-coil containing protein kinase 1 (ROCK1) in cervical cancer.
- To determine their impact on radiotherapy response in cervical cancer patients.
Main Methods:
- Analysis of miR-214-5p levels in 53 cervical cancer tissues based on radiotherapy response.
- Utilizing radiation-resistant cervical cancer cell lines to assess miR-214-5p's effect on radiosensitivity.
- Employing wound healing, transwell migration, clone formation, and in vivo assays.
Main Results:
- Low miR-214-5p levels were observed in patients with poor radiotherapy outcomes.
- Upregulation of miR-214-5p reduced migration and invasion in resistant cells.
- miR-214-5p directly targets ROCK1 mRNA, decreasing its expression and enhancing radiosensitivity in vitro and in vivo.
Conclusions:
- miR-214-5p regulates cervical cancer cell radiosensitivity.
- This regulation occurs via targeting and modulating ROCK1 mRNA expression.
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