Capacitive biosensors for label-free and ultrasensitive detection of biomarkers

Capacitive biosensors are label-free capacitors that can detect biomarkers with the outstanding advantages of simplicity, low cost, and ultrahigh sensitivity. A typical capacitive biosensor consists of a bioreceptor and a transducer, where the bioreceptor captures the biomarker to form a bioreceptor/biomarker conjugate and the transducer generates a detectable signal. In general, antibodies, aptamers, or proteins are exploited as the bioreceptor, while various electrodes including carbon electrodes (CEs), gold electrodes (AuEs), or interdigitated electrodes (IDEs) may serve as the transducer. Because the formation of bioreceptor/biomarker conjugates often leads to a change in capacitance, the capacitive signal is then employed for biomarker detection. This review summarizes recent advances in capacitive biosensors for the detection of biomarkers over the last five years. With a focus on the three common types of bioreceptors, i.e., antibodies, aptamers, and proteins, capacitive biosensors using CEs, AuEs, and IDEs as the transducers are discussed in detail. The immobilization of bioreceptors and signal amplification strategies are described to provide a robust overview of capacitive biosensors for biomarker detection. In addition, analytical methods and future prospects are given to support the application of capacitive biosensors.