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Related Experiment Video

Updated: Jul 22, 2025

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

Bioinspired engineered proteins enable universal anchoring strategy for surface functionalization.

Ziqian Zhao¹, Mingfei Pan¹, Wenshuai Yang¹

¹Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada.

Journal of Colloid and Interface Science

|July 24, 2023

Summary

This study engineered novel protein-based coatings using bovine serum albumin (BSA) and polyacrylates. These versatile biomaterials offer facile surface functionalization for advanced bio-applications.

Keywords:

Bovine serum albumin Coating Engineered protein Interfacial interactions Multifunction

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Area of Science:

Biomaterials Science
Surface Chemistry
Protein Engineering

Background:

Conventional bio-applications face challenges with complex coating preparation and limited on-demand functionalities.
Existing strategies often lack versatility for diverse protective, diagnostic, and therapeutic needs.

Purpose of the Study:

To develop a facile and versatile coating strategy inspired by natural structures.
To engineer novel polyacrylate-conjugated proteins for advanced surface functionalities.
To overcome limitations of conventional coating methods in bio-applications.

Main Methods:

Engineered novel polyacrylate-conjugated proteins using click chemistry.
Utilized bovine serum albumin (BSA) as a 'root' anchoring layer and polyacrylate as a 'leaf' functional layer.
Employed facile dip/spraying methods for universal substrate anchoring and characterized interactions via molecular force measurements.

Main Results:

Achieved universal anchoring onto organic and inorganic substrates with excellent stability in harsh conditions.
Demonstrated versatile on-demand functionalities including >99% biofouling resistance in complex biofluids.
Showcased pH-responsive performance and robust adhesion with various nanomaterials.

Conclusions:

Introduced a promising and facile strategy for imparting novel functionalities to diverse surfaces.
Leveraged synergistic anchoring of BSA with polyacrylate properties for enhanced performance.
Enabled engineering of natural proteins and biomaterials for biotechnical and nanotechnical applications.