Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.4K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.4K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.3K
5.3K
Exon Recombination02:32

Exon Recombination

3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic targeting of AREL1 in hepatic stellate cells attenuates MASH-related liver fibrosis.

Nature communications·2026
Same author

Effects of Different Vascular Lesions on the Clinical,Pathological, and Prognostic Features of Lupus Nephritis.

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae·2026
Same author

Editorial: AI for design and control of advanced robots.

Frontiers in robotics and AI·2026
Same author

Effects of hydrostatic pressure on epithelial dome formation and stability.

Soft matter·2026
Same author

Micro/nanoplastics and lithium iron phosphate at environmentally relevant dose triggers hepatic fibrosis: Unseen risks of global renewable energy.

Journal of hazardous materials·2026
Same author

Cumulative Social Determinants of Health Are Associated With Anxiety: A Weighted Quantile Sum Analysis.

Inquiry : a journal of medical care organization, provision and financing·2026

Related Experiment Video

Updated: Jul 22, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K

Alternative splicing: a bridge connecting NAFLD and HCC.

Kequan Xu1, Tiangen Wu1, Peng Xia1

  • 1Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, PR China; Clinical Medicine Research Center for Minimally Invasive Procedure of Hepatobiliary and Pancreatic Diseases of Hubei Province, Hubei, PR China.

Trends in Molecular Medicine
|July 24, 2023
PubMed
Summary

Non-alcoholic fatty liver disease (NAFLD) is a major risk factor for liver cancer (HCC). Alternative splicing (AS) and epigenetic changes in NAFLD progression to HCC are summarized, along with therapeutic strategies.

Keywords:
alternative splicingepigenetic modificationhepatocellular carcinomanon-alcoholic fatty liver diseasesplicing factor

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.8K

Related Experiment Videos

Last Updated: Jul 22, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.8K

Area of Science:

  • Hepatology and molecular biology
  • Cancer research
  • Epigenetics

Background:

  • Non-alcoholic fatty liver disease (NAFLD) is increasingly recognized as a significant risk factor for hepatocellular carcinoma (HCC).
  • Understanding the molecular mechanisms driving the progression from benign liver conditions to HCC is critical for developing effective prevention and treatment strategies.
  • Alternative splicing (AS) has emerged as a key player in the pathogenesis and transformation processes of liver diseases.

Purpose of the Study:

  • To review the role of alternative splicing (AS) in the progression of NAFLD to HCC.
  • To explore the impact of epigenetic modifications on AS and liver cell fate in NAFLD and HCC.
  • To summarize current and potential therapeutic approaches for NAFLD, HCC, and their early transitional stages.

Main Methods:

  • Literature review and synthesis of existing research on splicing factors, AS, and epigenetic modifications in NAFLD and HCC.
  • Analysis of how changes in splicing factor activity and epigenetic alterations influence liver cell fate.
  • Compilation of therapeutic strategies and drugs relevant to NAFLD, HCC, and early disease progression.

Main Results:

  • Alterations in splicing factor activity and aberrant AS are observed in NAFLD and HCC.
  • Epigenetic modifications, including DNA methylation, RNA methylation, histone modification, and protein phosphorylation, significantly impact AS and contribute to liver cell transformation.
  • Various therapeutic interventions show promise for managing NAFLD, HCC, and the intermediate stages of disease progression.

Conclusions:

  • Alternative splicing, modulated by epigenetic mechanisms, is a crucial factor in the development and progression of NAFLD to HCC.
  • Targeting splicing factors and epigenetic pathways presents a promising therapeutic avenue for liver cancer prevention and treatment.
  • A comprehensive understanding of these molecular events is essential for developing effective clinical strategies against NAFLD-associated HCC.