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DNA flow cytometry in human bladder carcinoma.

S D Fosså, E Thorud, E O Pettersen

    Pathology, Research and Practice
    |June 1, 1986
    PubMed
    Summary
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    DNA flow cytometry reveals cell kinetic fractions in bladder cancer. Tumor ploidy and S-phase fraction correlate with stage and grade, aiding prognosis and treatment evaluation.

    Area of Science:

    • Oncology
    • Cell Biology
    • Urology

    Background:

    • Bladder carcinoma presents a significant clinical challenge.
    • Understanding cell kinetics and DNA ploidy is crucial for prognosis.
    • Cytotoxic therapies aim to alter tumor cell proliferation.

    Purpose of the Study:

    • To evaluate cell kinetic fractions (G0/G1, S, G2+M) using DNA flow cytometry (DNA FCM).
    • To correlate these parameters with tumor characteristics and treatment response in bladder cancer.
    • To assess the utility of DNA FCM for prognosis and therapy effect evaluation.

    Main Methods:

    • DNA flow cytometry (DNA FCM) was performed on 102 untreated bladder carcinoma biopsies.
    • 25 biopsies from patients treated at least 3 months prior were also analyzed.

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  • Cell kinetic fractions and DNA stemline ploidy were quantified.

    • Main Results:

    • Non-diploid DNA stemlines were more frequent in high T category and high-grade tumors.
    • A high S-phase fraction (>10%) correlated with advanced clinical stage and histological grade.
    • Post-treatment tumor ploidy in recurrent/residual cases mirrored untreated patient distributions.
    • Muscle-infiltrating tumors often retained stemline ploidy after treatment.

    Conclusions:

    • DNA FCM analysis of cell kinetics and ploidy provides valuable prognostic information in bladder cancer.
    • These parameters can help assess the effectiveness of cytotoxic therapies.
    • DNA FCM offers a potential tool for personalized treatment strategies in bladder carcinoma.