Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Electrostatic Boundary Conditions in Dielectrics01:27

Electrostatic Boundary Conditions in Dielectrics

When an electric field passes from one homogeneous medium to another, crossing the boundary between the two mediums imparts a discontinuity in the electric field. This results in electrostatic boundary conditions that depend on the type of mediums the field propagates through.
Consider a case where both the mediums across a boundary are two different dielectric materials. Recall that the electric field and electric displacement are proportional and related through the material's permittivity.
The Electrical Double Layer01:30

The Electrical Double Layer

In the region where two bulk phases meet, an intricate electric charge distribution arises due to charge transfer, ion adsorption, molecular orientation, and charge distortion. This complex distribution is commonly referred to as the electrical double layer.When a solid electrode interfaces with ions in an electrolyte solution, the speed of electron transfer dictates the rates of oxidation and reduction. The electrode acquires a charge through the escape of atoms into the solution as cations or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leptin drives osteoarthritis pain through sensory neuron reprogramming independent of cartilage signaling.

bioRxiv : the preprint server for biology·2026
Same author

Synthetic Mechanogenetic Gene Circuit Response in Human Induced Pluripotent Stem Cell-Derived Chondrocytes Is Altered by Matrix Stiffness.

Journal of biomedical materials research. Part A·2026
Same author

What's New in Biologics.

The Journal of bone and joint surgery. American volume·2026
Same author

Adipose-driven complement-lipid reprogramming controls nociceptive vulnerability in obesity-associated osteoarthritis.

bioRxiv : the preprint server for biology·2026
Same author

Tendon Omics: A Survey-Based Perspective on Obstacles and Opportunities.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

Strain-conditioned spatial gene expression in female mouse bone under physiological and non-physiological strain distribution.

JBMR plus·2026

Related Experiment Video

Updated: Jul 23, 2026

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
10:08

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture

Published on: October 21, 2009

21.6K

Tendon-derived matrix crosslinking techniques for electrospun multi-layered scaffolds.

Thomas L Jenkins1, Paula A Sarmiento Huertas1, Kentaro Umemori1

  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.

Journal of Biomedical Materials Research. Part A
|July 25, 2023
PubMed
Summary

Crosslinking tendon-derived matrix (TDM) to poly(ε-caprolactone) (PCL) scaffolds using UV or EDC methods enhances cell attachment and tendon-like matrix synthesis. This strategy improves TDM retention on scaffolds for better tendon repair.

Keywords:
carbodiimidecollagenextracellular matrixhuman adipose stem cellsmesenchymal stem celltissue engineeringultraviolet radiation

More Related Videos

Postproduction Processing of Electrospun Fibres for Tissue Engineering
15:52

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Published on: August 9, 2012

18.2K
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

9.9K

Related Experiment Videos

Last Updated: Jul 23, 2026

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
10:08

Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture

Published on: October 21, 2009

21.6K
Postproduction Processing of Electrospun Fibres for Tissue Engineering
15:52

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Published on: August 9, 2012

18.2K
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

9.9K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tendon tears often result in incomplete healing and fibrosis, necessitating improved repair strategies.
  • Tissue engineering approaches utilize cell-seeded scaffolds with biomimetic factors to enhance tendon repair.
  • Effective retention of biomimetic factors on scaffolds is crucial for maximizing therapeutic benefits and minimizing risks.

Purpose of the Study:

  • To evaluate cross-linking methods for enhancing the retention of tendon-derived matrix (TDM) on electrospun poly(ε-caprolactone) (PCL) scaffolds.
  • To assess the impact of cross-linking on human adipose stem cell (hASC) behavior and tendon-like matrix synthesis.

Main Methods:

  • Tested ultraviolet (UV) and carbodiimide (EDC:NHS:COOH) cross-linking methods on PCL films with TDM.
  • Evaluated TDM retention, hASC spreading, and mechanical properties (modulus, stiffness) of cross-linked scaffolds.
  • Assessed hASC-induced tendon-like differentiation and analyzed scaffold composition using Fourier transform infrared spectroscopy.

Main Results:

  • No tested cross-linking method significantly retained more TDM than coating alone, but UV and EDC methods improved hASC spreading.
  • UV-crosslinked scaffolds exhibited increased modulus and stiffness compared to untreated or TDM-coated PCL scaffolds.
  • hASCs demonstrated enhanced spreading on UV-crosslinked scaffolds, and cross-linking did not alter characteristic tendon matrix peaks.

Conclusions:

  • Cross-linking TDM to electrospun PCL scaffolds improves tendon-like matrix synthesis.
  • UV or EDC cross-linking offers a viable strategy for enhancing TDM retention on PCL scaffolds.
  • This approach holds promise for improving outcomes in tendon tissue engineering and repair.