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Paracholeplasma manati sp. nov. isolated from Florida manatees (Trichechus manatus latirostris)

Dmitriy V Volokhov1, Vyacheslav A Furtak1, Tatiana A Zagorodnyaya1

  • 1Center for Biologics Evaluation and Research, US Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA.

International Journal of Systematic and Evolutionary Microbiology
|July 25, 2023
PubMed

Insights

Four new species of Paracholeplasma bacteria were discovered on Florida manatees. These novel bacteria, named Paracholeplasma manati, are genetically and phenotypically distinct from known species.

Area of Science:

  • Microbiology
  • Mycoplasmology
  • Marine Mammal Health

Background:

  • The genus Paracholeplasma comprises bacteria that can inhabit various animal hosts.
  • Understanding the diversity of microbial communities associated with marine mammals is crucial for assessing their health.

Purpose of the Study:

  • To isolate and characterize novel bacterial strains from Florida manatees.
  • To determine the phylogenetic and genomic relationships of these novel strains within the genus Paracholeplasma.

Main Methods:

  • Isolation of bacteria from Florida manatee skin.
  • Phenotypic characterization including growth conditions and biochemical tests.
  • Genetic analysis using 16S rRNA, rpoB, gyrB, and parE gene sequencing.
  • Whole-proteome phylogenetic analysis.
  • Genomic analysis including ANI and dDDH calculations.

Main Results:

  • Four novel Paracholeplasma strains (OakleyT, Holly, Lorelei, Ariel) were isolated from manatee skin.
  • These strains exhibited unique phenotypic characteristics, including acid production from glucose.
  • Phylogenetic and genomic analyses confirmed these strains represent a novel species, Paracholeplasma manati sp. nov., distinct from known Paracholeplasma species.

Conclusions:

  • The discovery of Paracholeplasma manati expands the known diversity of the genus Paracholeplasma.
  • This finding highlights the unique microbiome associated with Florida manatees.
  • Further research is warranted to understand the ecological role and potential impact of P. manati on manatee health.
Keywords:
16S rRNAAcholeplasmaParacholeplasmamanateemarine mammalsphylogeny

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