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Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Non-typhoidal salmonella contamination along the pork value chain in a rural East African setting: a cross-sectional
Cianjo M Gichuyia1,2, Lian F Thomas2,3, Christine Makena1,2
1Department of Public Health, Pharmacology and Toxicology, University of Nairobi, P. O. Box 29053-00625, Kangemi, Kenya.
Background:
Non-typhoidal Salmonella (NTS) is a serious foodborne pathogen that has previously been isolated from pigs presented for slaughter in a rural pork value chain in western Kenya.
Methods:
To understand varying NTS contamination along the value chain we assessed prevalence at slaughter, transport and retail. Suspect isolates from culture were confirmed using matrix-assisted laser desorption-ionization time of flight mass spectrometry.
Results:
Prevalence on pig carcasses, meat transportation containers, retailed raw and cooked pork and accompanying side salads was 18.1%, 23.9%, 28.0%, 1.9% and 8.6%, respectively.
Conclusion:
NTS contamination is propagated along the pork value chain in rural western Kenya, demonstrating the need for improved hygiene measures to prevent human exposure.
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