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Related Experiment Video

Updated: Jul 21, 2025

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Published on: May 19, 2019

Long-term evolution of proliferating cells using the eVOLVER platform.

Daniel García-Ruano^1,2, Akanksha Jain^1,2, Zachary J Heins³

¹Institute of Genetics and Development of Rennes, CNRS UMR 6290 - University of Rennes 1, Rennes, France.

|July 26, 2023

Summary

This summary is machine-generated.

This study enhances the eVOLVER system for long-term experimental evolution. The upgraded framework offers a versatile and automated platform for studying evolutionary processes in unicellular organisms.

Keywords:

adaptation eVOLVER experimental evolution turbidostat yeast

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Published on: September 19, 2019

Area of Science:

Evolutionary biology
Microbiology
Systems biology

Background:

Experimental evolution with unicellular organisms reveals evolutionary principles and biological functions.
Automated systems like eVOLVER facilitate multiplexed experimental evolution.
Previous eVOLVER limitations included difficulties in long-term parallel culture maintenance and lack of specific tools.

Purpose of the Study:

To significantly upgrade the eVOLVER framework for enhanced long-term experimental evolution.
To address limitations in maintaining and comparing parallel evolving cultures.
To provide a more versatile and automated platform for laboratory evolution assays.

Main Methods:

Major modifications to experimental methodology, hardware, and software of the eVOLVER system.
Development of a new stand-alone protocol for improved usability.
Implementation of a closed-loop system for fully automated laboratory evolution.

Main Results:

The upgraded eVOLVER framework enables robust, long-term maintenance and comparison of parallel evolving cultures.
Enhanced automation and control over experimental conditions.
Improved versatility and reduced user intervention for extended assays.

Conclusions:

The enhanced eVOLVER system is a powerful and unparalleled setup for long-term experimental evolution studies.
The modifications provide greater reliability and efficiency in evolutionary experiments.
Facilitates deeper insights into evolutionary mechanisms and biological system architectures.