Free amino acids in bee hive product (propolis) as identified and quantified by gas-liquid chromatography

Propolis is a natural resinous product collected by honey bees and containing, among other biochemical constituents, a variety of free amino acids. Acid extraction and quantification of these amino acids by gas-liquid chromatography reveals that their total concentration in this honey bee product is over 40% w/w, and that arginine and proline constitutes over 50% of the crude acid extract. As propolis was shown to stimulate mammalian tissue regeneration, we suggest that the physiological significance of arginine in the propolis product lies in its ability to stimulate mitosis and to enhance protein biosynthesis, and that the biochemical importance of proline in it, stems from its capability to promote build-up of collagen and elastin, two essential components in the matrix of connective tissues.