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Chromatographic method for dacarbazine quantification in skin permeation experiments.

Camila O Cardoso1, Thaís Y Uwai1, Taís Gratieri1

  • 1Laboratory of Food, Drugs, and Cosmetics (LTMAC), School of Health Sciences, University of Brasília, 70910-900 Brasília, DF, Brazil.

Journal of Pharmaceutical and Biomedical Analysis
|July 26, 2023
PubMed
Summary

A new selective chromatographic method was validated for quantifying dacarbazine (DTIC) in skin samples. This method is crucial for developing less toxic topical melanoma treatments by accurately measuring drug levels in skin permeation studies.

Keywords:
HPLC-UVMelanomaSkin cancerTopicalValidation

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Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Dermatology

Background:

  • Dacarbazine (DTIC) is a key chemotherapy for melanoma.
  • Topical DTIC offers a potentially less toxic and more comfortable alternative to systemic treatment.
  • Accurate quantification of DTIC in skin is essential for topical formulation development but challenging due to skin interferents.

Purpose of the Study:

  • To validate a selective chromatographic method for determining DTIC concentrations in skin.
  • To support the development of novel topical dacarbazine formulations for melanoma treatment.
  • To enable reliable skin permeation studies of DTIC.

Main Methods:

  • High-performance liquid chromatography (HPLC) using a reversed-phase C18 column.
  • Gradient elution with methanol and a sodium phosphate buffer (0.01 mol/L, pH 6.5) at 1.0 mL/min.
  • Detection of DTIC at 364 nm.

Main Results:

  • The method demonstrated high selectivity against skin interferents.
  • Linearity was confirmed in the 1.0-15.0 μg/mL range (r=0.9995).
  • The method was precise (CV < 3.8%), accurate (91-112% recovery), and sensitive (LOD=0.10 μg/mL, LOQ=0.30 μg/mL).
  • Successful application in in vitro skin permeation studies.

Conclusions:

  • The validated chromatographic method is suitable for analyzing DTIC in skin samples.
  • This analytical tool facilitates the design and evaluation of topical dacarbazine formulations.
  • The method supports advancements in topical melanoma therapy.