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MicroRNA-765 is upregulated in myelodysplastic syndromes and induces apoptosis via PLP2 inhibition in leukemia cells

Seong-Ho Kang1, Ji Seon Choi2

  • 1Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Korea.

Blood Research
|July 26, 2023
PubMed
Abstract

Insights

MicroRNA-765 (miR-765) is upregulated in myelodysplastic syndromes (MDS). This upregulation inhibits leukemia cell proliferation and induces apoptosis by downregulating proteolipid protein 2 (PLP2), suggesting a role in MDS pathogenesis.

Area of Science:

  • Hematology
  • Molecular Biology
  • Epigenetics

Background:

  • MicroRNA (miRNA) dysregulation is implicated in hematologic malignancies.
  • miR-765 has been shown to inhibit cell proliferation and induce apoptosis by targeting proteolipid protein 2 (PLP2).
  • This study investigates the role of miR-765 in myelodysplastic syndromes (MDS).

Purpose of the Study:

  • To investigate miR-765 dysregulation in myelodysplastic syndromes (MDS).
  • To determine the functional role of miR-765 in leukemia cells.
  • To identify the molecular targets of miR-765 in MDS.

Main Methods:

  • Comparative analysis of miR-765 expression in 65 MDS patients and 11 controls using RT-qPCR.
  • In vitro cell proliferation and apoptosis assays in leukemia cells transfected with miR-765 mimic.
  • Validation of miR-765 targets (PLP2) using RT-qPCR and western blotting.

Main Results:

  • miR-765 levels were significantly upregulated (10.2-fold) in MDS patients compared to controls.
  • Elevated miR-765 was more prevalent in refractory cytopenia with multilineage dysplasia.
  • miR-765 mimic transfection inhibited leukemia cell proliferation and induced apoptosis, with PLP2 identified as a direct target.

Conclusions:

  • Upregulation of miR-765 in MDS induces apoptosis through PLP2 downregulation.
  • miR-765 may play a significant role in the pathogenesis of myelodysplastic syndromes.
  • These findings highlight miR-765 as a potential therapeutic target in MDS.
Keywords:
ApoptosisDeregulationMDSPathogenesismicroRNA

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