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Sinomenine regulates circTRPM7-related pathway to inhibit gastric cancer cell growth and metastasis

Jingwei Yan1, Jingqing Yang2, Huifen Shen1

  • 1Department of Traditional Chinese Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.

Chemical Biology & Drug Design
|July 26, 2023
PubMed

Insights

Sinomenine exhibits antitumor properties in gastric cancer by regulating the circTRPM7/microRNA-145-5p/PBX3 pathway, inhibiting cancer cell growth and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Sinomenine demonstrates antitumor potential in various cancers, including gastric cancer.
  • Circular RNAs (circRNAs) are implicated in gastric cancer progression, but their interaction with Sinomenine is unclear.

Purpose of the Study:

  • To investigate whether Sinomenine exerts its antitumor effects in gastric cancer by modulating circRNA pathways.
  • To elucidate the specific molecular mechanisms involving circTRPM7, microRNA-145-5p, and PBX3.

Main Methods:

  • Quantitative real-time PCR for gene expression analysis.
  • Cellular assays (MTT, colony formation, EdU, Transwell, wound-healing, flow cytometry) for proliferation, migration, invasion, and apoptosis.
  • Western blot for protein analysis and dual-luciferase/RIP assays for molecular interactions.

Main Results:

  • Sinomenine treatment reduced circTRPM7 expression in gastric cancer cells.
  • Overexpression of circTRPM7 promoted growth and metastasis, while circTRPM7 sponged miR-145-5p, which reversed these effects.
  • miR-145-5p targeted PBX3, and PBX3 knockdown counteracted miR-145-5p's tumor-suppressive actions.

Conclusions:

  • Sinomenine exerts an antitumor role in gastric cancer.
  • This effect is mediated through the circTRPM7/miR-145-5p/PBX3 axis, highlighting a novel therapeutic pathway.
Keywords:
PBX3SinomeninecircTRPM7gastric cancermiR-145-5p

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