MFNG is an independent prognostic marker for osteosarcoma

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Osteosarcoma (OS) is a prevalent bone cancer in children and adolescents with a challenging prognosis.
• Identifying reliable prognostic markers is crucial for improving patient outcomes in OS.

Purpose of the Study:

• To identify and validate prognostic markers for osteosarcoma (OS).
• To investigate the role of Manic Fringe (MFNG) as a prognostic factor in OS.

Main Methods:

• Utilized Gene Expression Omnibus (GEO) dataset (GSE39058) for mRNA and clinical data of OS patients.
• Employed Kaplan-Meier curves and Cox regression analysis to identify prognosis-related genes (PRGs).
• Performed Gene Ontology (GO) analysis and in vitro assays (CCK-8, apoptosis) to assess MFNG function.

Main Results:

• Manic Fringe (MFNG) emerged as a key gene strongly associated with OS prognosis and clinical features.
• Elevated MFNG expression correlated with poorer disease-free survival and was an independent adverse prognostic factor.
• MFNG overexpression promoted cell proliferation and inhibited apoptosis in U2OS osteosarcoma cells.

Conclusions:

• MFNG expression negatively correlates with osteosarcoma progression.
• MFNG serves as an independent adverse prognostic factor for disease-free survival in OS patients.
• MFNG influences osteosarcoma cell proliferation and apoptosis.