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Updated: Jul 21, 2025
Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models
Published on: July 7, 2016
Whole-body bioluminescence imaging of T-cell response in PDAC models
Roisin McMorrow1,2,3, Giorgia Zambito1,2, Alex Nigg4
1Erasmus Medical Centre, Department of Radiology and Nuclear Medicine, Rotterdam, Netherlands.
Introduction:
The location of T-cells during tumor progression and treatment provides crucial information in predicting the response in vivo.
Methods:
Here, we investigated, using our bioluminescent, dual color, T-cell reporter mouse, termed TbiLuc, T-cell location and function during murine PDAC tumor growth and checkpoint blockade treatment with anti-PD-1 and anti-CTLA-4. Using this model, we could visualize T-cell location and function in the tumor and the surrounding tumor microenvironment longitudinally. We used murine PDAC clones that formed in vivo tumors with either high T-cell infiltration (immunologically 'hot') or low T-cell infiltration (immunologically 'cold').
Results:
Differences in total T-cell bioluminescence could be seen between the 'hot' and 'cold' tumors in the TbiLuc mice. During checkpoint blockade treatment we could see in the tumor-draining lymph nodes an increase in bioluminescence on day 7 after treatment.
Conclusions:
In the current work, we showed that the TbiLuc mice can be used to monitor T-cell location and function during tumor growth and treatment.
