Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Blood Vessels01:07

Development of Blood Vessels

641
The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
641
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

5.6K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.6K
Liver Regeneration01:24

Liver Regeneration

3.3K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.3K
Hepatic Portal System01:21

Hepatic Portal System

1.5K
The hepatic portal system, a critical part of our circulatory framework, transports nutrient-laden, deoxygenated blood from the gastrointestinal tract and spleen to the liver. This ingenious system plays an indispensable role in maintaining our body's metabolic equilibrium.
At its core, the hepatic portal vein is the result of a confluence of the superior and inferior mesenteric veins along with the splenic vein. Each of these veins has a unique role. The superior mesenteric vein is...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraoperative irreversible electroporation for margin accentuation in locally advanced intrahepatic cholangiocarcinoma: a case series.

Frontiers in surgery·2026
Same author

DNA methylation-based classification uncovers acinar and ductal origins for KPC-derived PDAC cell lines.

Cancer letters·2026
Same author

Luminal-basal stratification of the native human pancreatic duct is differentially represented in pancreatic cancers.

Gut·2026
Same author

omicsGMF: a multi-tool for dimensionality reduction, batch correction and imputation in bulk- and single-cell proteomics.

Nature communications·2026
Same author

GLP1-E2 therapy delays autoimmune diabetes in late-stage prediabetic NOD mice and potentiates low-dose anti-CD3 therapy for enhanced disease protection.

Diabetologia·2026
Same author

Increased Protein Synthesis With Reduced Endoplasmic Reticulum Stress Defines a Specific Adaptation in Pancreatic Acinar Metaplasia.

Gastro hep advances·2026

Related Experiment Video

Updated: Jul 21, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

468

Axon guidance genes control hepatic artery development.

Lila Gannoun1, Catalina De Schrevel1, Morgane Belle2

  • 1de Duve Institute, Université Catholique de Louvain, Avenue Hippocrate 75, Brussels 1200, Belgium.

Development (Cambridge, England)
|July 27, 2023
PubMed
Summary

Researchers identified new signaling pathways crucial for liver vasculature development. Defective ROBO2/SLIT2 signaling impairs hepatic artery formation, impacting liver regeneration after injury.

Keywords:
Liver developmentLiver mesenchymeSlit-Robo signalling

More Related Videos

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
08:14

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Published on: May 20, 2015

8.7K
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

17.7K

Related Experiment Videos

Last Updated: Jul 21, 2025

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

468
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
08:14

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Published on: May 20, 2015

8.7K
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

17.7K

Area of Science:

  • Developmental Biology
  • Hepatology
  • Vascular Biology

Background:

  • Liver development involves interdependent morphogenesis of bile ducts, portal mesenchyme, and hepatic arteries.
  • The precise orchestration of these interdependent developmental processes remains incompletely understood.

Purpose of the Study:

  • To elucidate the mechanisms governing hepatic artery formation and its interdependence with other portal area structures.
  • To identify key intercellular signaling pathways involved in liver vasculature development and regeneration.

Main Methods:

  • Utilized 2D and 3D imaging techniques to visualize portal area morphogenesis.
  • Investigated intercellular signaling by examining gene expression, focusing on axon guidance genes.
  • Employed tissue-specific gene inactivation in mouse models to assess the roles of RGMA/NEO1 and ROBO2/SLIT2 signaling pathways.

Main Results:

  • Described the organization of portal mesenchymal cells into hepatic arteries.
  • Identified dynamic expression of axon guidance genes in developing bile ducts and mesenchyme.
  • Demonstrated that ROBO2/SLIT2 signaling in the portal mesenchyme is essential for hepatic artery tunica media maturation.
  • RGMA/NEO1 signaling was found to be dispensable for portal area development.

Conclusions:

  • Discovered that ROBO2/SLIT2 signaling is critical for proper hepatic artery development.
  • Showed that impaired hepatic artery maturation affects liver regeneration and increases tissue damage post-hepatectomy.
  • Identified novel molecular players involved in liver vasculature development and regeneration.