Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intrinsic capacity and the trajectory of ischemic heart disease and its complications: the mediating role of insulin resistance indices.

Maturitas·2026
Same author

Timing and regimen of antiretroviral therapy and all-cause mortality in people with HIV: A time-to-event mediation analysis via opportunistic infection.

International journal of antimicrobial agents·2026
Same author

Neutrophil membrane-mediated biomimetic nanoparticle for co-delivery of icariin and siORMDL3 attenuates asthma exacerbated by respiratory syncytial virus.

Journal of nanobiotechnology·2026
Same author

Whole β-glucan particles modulate BCG-induced macrophage and CD4<sup>+</sup> T-cell responses via the Dectin-1-JAK1/STAT1 pathway.

Communications biology·2026
Same author

Remodeling the pathological airway: advanced nanotechnology for diagnostics and therapeutics in cystic fibrosis.

Journal of nanobiotechnology·2026
Same author

Rational design of low-risk and high-efficiency β-triketone herbicide alternatives via integrative computational toxicology and NGBoost modeling.

Molecular diversity·2026

Related Experiment Video

Updated: Jul 21, 2025

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
12:50

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Published on: June 9, 2014

14.6K

A rapid screening platform for antibiotic susceptibility testing based on a simple colorimetric method.

Rui Zhao1, Yubin Shen1, Chenyu Zhao1

  • 1Key Laboratory of Textile Fiber and Products Ministry of Education, School of Materials Science and Engineering, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials & Application, Wuhan Textile University, Wuhan, 430200, China. luzhentan@wtu.edu.cn.

The Analyst
|July 27, 2023
PubMed
Summary

A new rapid antibiotic susceptibility testing (AST) platform uses nanofiber membranes and cell counting Kit-8 (CCK8) colorimetry for faster results. This method significantly reduces detection time, offering a promising tool for clinical practice.

More Related Videos

A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
09:39

A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries

Published on: December 27, 2016

17.8K
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
10:50

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Published on: April 10, 2016

18.6K

Related Experiment Videos

Last Updated: Jul 21, 2025

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
12:50

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Published on: June 9, 2014

14.6K
A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
09:39

A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries

Published on: December 27, 2016

17.8K
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
10:50

Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials

Published on: April 10, 2016

18.6K

Area of Science:

  • Biotechnology
  • Clinical Diagnostics
  • Microbiology

Background:

  • Antibiotic resistance necessitates rapid antibiotic susceptibility testing (AST) in clinical settings.
  • Current AST methods can be time-consuming, delaying appropriate treatment.
  • Developing faster diagnostic tools is crucial for effective bacterial infection management.

Purpose of the Study:

  • To develop and validate a novel, rapid screening platform for antibiotic susceptibility testing (AST).
  • To assess the platform's efficiency in bacterial enrichment and accurate cell counting.
  • To demonstrate the platform's utility with clinically relevant samples.

Main Methods:

  • Utilized a nanofiber membrane for bacterial enrichment and separation from culture media.
  • Employed cell counting Kit-8 (CCK8) colorimetry, correlating formazan absorbance with bacterial load.
  • Validated the method against traditional AST techniques using clinical samples.

Main Results:

  • Achieved a total detection time of 7-9 hours, significantly faster than traditional methods.
  • Established a linear relationship between CCK8 formazan absorbance and bacterial count.
  • Demonstrated identical antibiotic susceptibility evaluation results compared to conventional methods.
  • Achieved a low limit of detection of 10 CFU mL-1.
  • Successfully applied the platform to urine, blood, and wound fluid samples.

Conclusions:

  • The developed nanofiber membrane-based platform offers a rapid and reliable method for AST.
  • This approach effectively eliminates antibiotic interference, ensuring accurate results.
  • The platform meets the criteria for rapid testing and shows significant promise for clinical application.