A model study on mode of bonding of cisplatin with DNA

The interaction of cis-[Pt(NH3)2Cl2] with nucleosides, as constituents of DNA, was studied by spectrophotometric, 13C NMR and CNDO/2 methods. The apparent stability constants of the complexes formed and the initial rate of formation were determined. The sites of binding of nucleoside (or nucleotide) with Cisplatin were ascertained and the electronic structure of the model coordination compounds of Cisplatin and guanine was calculated by MO approach. On the basis of the obtained results, the mechanism of the antitumour action of Cisplatin was discussed, and a new possible mode of bonding of Cisplatin with DNA was proposed.