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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Related Experiment Video

Updated: Jul 21, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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Single-Stranded RNA Origami-Based Epigenetic Immunomodulation.

Kun Dai1, Chen Gong2, Yang Xu3

  • 1School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, Zhangjiang Institute for Advanced Study and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Nano Letters
|July 27, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a transcription method to precisely add functional molecules to RNA nanostructures. This creates advanced RNA origami with new biomedical capabilities for disease treatment.

Keywords:
Enhanced interferon chemotherapyEpigenetic modificationNucleic acid nanotechnologySingle-stranded RNA origami

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • RNA nanostructures offer versatile platforms for molecular applications.
  • Quantitative integration of nucleoside analogues into RNA nanostructures is challenging and underexplored.
  • Understanding the impact of nucleoside analogues on RNA nanostructure function is crucial.

Purpose of the Study:

  • To develop a transcription-based method for controlled integration of nucleoside analogues into RNA nanostructures.
  • To investigate the effects of integrated nucleoside analogues on the structure, stability, and function of RNA nanostructures.
  • To explore the potential of functionalized RNA nanostructures for biomedical applications.

Main Methods:

  • A transcription-based approach was utilized to synthesize a 2000-nucleotide single-stranded RNA (ssRNA) origami nanostructure.
  • Multiple nucleoside analogues were controllably integrated into the ssRNA origami at the molecular level.
  • The morphology, biostability, and biomedical functions of the integrated ssRNA origami were analyzed.

Main Results:

  • The integration of nucleoside analogues did not compromise the morphology or biostability of the ssRNA origami.
  • Epigenetic nucleoside analogues conferred innate immune recognition and regulatory functions.
  • Therapeutic nucleoside analogues enhanced synergistic effects on tumor cell killing.
  • The developed method allows for quantitative integration of functional nucleoside analogues.

Conclusions:

  • A novel transcription-based method enables precise molecular-level integration of functional nucleoside analogues into RNA nanostructures.
  • Functionalized RNA nanostructures exhibit preserved structural integrity and enhanced biomedical functionalities.
  • This approach paves the way for developing multifunctional RNA origamis for diverse applications, particularly in biomedicine.