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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
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Smartphone-based pH responsive 3-channel colorimetric biosensor for non-enzymatic multi-antibiotic residues
Xiaotian Zhou1, Siyuan Fu1, Jiahua Li1
1Sanya Institute of Nanjing Agricultural University, MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Food Chemistry
|July 27, 2023
Summary
This study presents a smartphone-based biosensor for rapid, on-site detection of multiple antibiotic residues in milk. The innovative system ensures food safety by identifying harmful contaminants like oxytetracycline and kanamycin.
Area of Science:
- Food Safety
- Analytical Chemistry
- Biosensor Technology
Background:
- Antibiotic residues in animal-derived food present significant food safety and human health risks.
- Accurate and rapid detection methods for multiple antibiotic residues are crucial for effective monitoring.
Purpose of the Study:
- To develop a smartphone-based, pH-responsive colorimetric biosensor for simultaneous detection of non-enzymatic multi-antibiotic residues in milk.
- To provide a portable and on-site detection solution for practical food safety applications.
Main Methods:
- A 3-channel colorimetric biosensor system was constructed utilizing magnetic separation and enrichment.
- Polydopamine-silver nanoparticles with pH-responsive properties were employed for sensitive detection.
- Smartphone integration enabled portable and on-site analysis.
Main Results:
- Simultaneous detection of oxytetracycline, kanamycin, and streptomycin was achieved.
- Linear detection ranges were established from 1-10^5 pg/mL.
- Low detection limits were reported: 0.085 pg/mL for oxytetracycline, 0.168 pg/mL for kanamycin, and 0.307 pg/mL for streptomycin.
- The system's practicality was validated using challenging milk samples.
Conclusions:
- The developed smartphone-based biosensor offers a sensitive, rapid, and portable method for multi-antibiotic residue analysis in milk.
- This technology holds significant promise for enhancing food safety guarantees and on-site monitoring.
- The system demonstrates broad applicability in multi-antibiotic residue detection within the food industry.
