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Factors Influencing Microbial Growth: pH

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Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...

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Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

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Fluctuating pH for efficient photomixotrophic succinate production.

Tanner R Treece¹, Marissa Tessman², Robert S Pomeroy³

¹Department of Chemistry, University of California, Davis, Davis, CA, 95616, USA.

Metabolic Engineering

|July 27, 2023

Summary

Photomixotrophy enhances cyanobacterial bioproduction by supplementing autotrophic metabolism with sugar. This study achieved 5.0 g L^-1 succinate production using engineered cyanobacteria under fluctuating pH conditions.

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Area of Science:

Synthetic biology
Metabolic engineering
Biochemical production

Background:

Cyanobacteria are promising photosynthetic hosts for bioproduction, but photoautotrophic efficiency is limited.
Photomixotrophy, combining autotrophic and heterotrophic metabolism, enhances bioproduction by utilizing multiple energy sources.
Succinate is a valuable industrial chemical with applications in pharmaceuticals and biopolymers.

Purpose of the Study:

To investigate the photomixotrophic production of succinate using engineered cyanobacteria.
To address the challenges of contradictory pH requirements for glucose import and succinate export.

Main Methods:

Engineered cyanobacteria were cultivated under photomixotrophic conditions.
Fluctuating pH cycles were implemented to optimize substrate import and product export.
Bicarbonate was used as a carbon source for CO2 fixation.

Main Results:

Engineered strains produced succinate under fluctuating pH conditions.
A succinate titer of 5.0 g L^-1 was achieved after 10 days in shake flask conditions.
Efficient glucose import was observed at neutral pH.

Conclusions:

Photomixotrophic production is a viable strategy for enhancing succinate yield in cyanobacteria.
Dynamic pH control can overcome transport limitations in engineered metabolic pathways.
This approach shows potential for large-scale industrial succinate production.