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Updated: Jul 21, 2025

Protocol for Long Duration Whole Body Hyperthermia in Mice
Published on: August 25, 2012
Potentiation of Anticancer Activity of G2/M Blockers by Mild Hyperthermia
Yuya Tagawa1, Hiroshi Sakagami2, Sei-Ichi Tanuma3
1Division of Oral and Maxillofacial Surgery, Meikai University School of Dentistry, Saitama, Japan.
Background/Aim:
Hyperthermia (HT), combined with chemotherapy, has been used to treat various types of cancer. This study aimed to investigate the HT-sensitivity of malignant and non-malignant cells, and then evaluate the combination effect of docetaxel (DTX) and a newly synthesized chromone derivative (compound A) with HT.
Materials And Methods:
The number of viable cells was determined using the MTT method. Cell cycle distribution was analyzed using a cell sorter, and DNA fragmentation pattern was detected using agarose gel electrophoresis.
Results:
Among 12 cultured cells, oral squamous cell carcinoma (OSCC), especially Ca9-22 cells, and myelogenous leukemia cells showed higher sensitivity to HT than lung carcinoma and glioblastoma cell lines, while normal oral cells were the most resistant. Cytotoxicity of DTX on Ca9-22 cells was maximum at 41-42°C and 45~60 min exposure to HT. DXT, compound A, and HT induced G2/M arrest of Ca-22 cells. Mild HT enhanced the DTX- and compound A-induced subG1 arrest, in a synergistic fashion.
Conclusion:
The combination G2/M blockers and mild-HT can potentially be used for the treatment of OSCC.
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