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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Related Experiment Video

Updated: Jul 21, 2025

Author Spotlight: Simplifying Genome-Wide Plasmid Library Construction Using CRISPRmass
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Published on: May 17, 2024

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The expanded CRISPR toolbox for constructing microbial cell factories.

Yuxi Teng1, Tian Jiang1, Yajun Yan1

  • 1School of Chemical, Materials and Biomedical Engineering, College of Engineering, University of Georgia, Athens, GA 30602, USA.

Trends in Biotechnology
|July 27, 2023
PubMed
Summary

CRISPR technology advancements significantly enhance microbial cell factory (MCF) construction. Expanded CRISPR toolboxes enable precise genetic engineering for producing valuable compounds from simple carbon sources.

Keywords:
CRISPR toolboxbase editingmetabolic engineeringmicrobial cell factorysynthetic metabolonstunable regulation

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Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Microbial cell factories (MCFs) are crucial for converting low-cost carbon sources into valuable compounds.
  • The CRISPR/Cas9 system has transformed genome editing in MCF construction due to its programmability.

Purpose of the Study:

  • To summarize recent advancements in CRISPR toolbox development for MCF construction.
  • To highlight the applications of expanded CRISPR systems in gene editing, transcriptional regulation, and enzyme modulation.

Main Methods:

  • Review of novel CRISPR systems and engineered Cas effectors.
  • Exploration of CRISPR applications for multi-level regulation and double-strand break-free editing.
  • Analysis of CRISPR's role in pathway construction, enzyme engineering, and metabolic control.

Main Results:

  • The expanded CRISPR toolbox offers enhanced programmability and precision for MCF engineering.
  • New CRISPR systems facilitate multi-level regulation and enable gene editing without double-strand breaks.
  • CRISPR tools effectively support pathway construction, enzyme optimization, and metabolic burden management in MCFs.

Conclusions:

  • The evolving CRISPR toolbox is a powerful asset for advancing microbial cell factory design and applications.
  • Future development and integration of CRISPR technologies promise further innovations in synthetic biology and biomanufacturing.