Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Energy to Drive Translocation01:37

Energy to Drive Translocation

2.1K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanism-informed neuroprotection in acute ischemic stroke treated with thrombectomy: a systematic review and meta-analysis of randomized controlled trials.

Frontiers in pharmacology·2026
Same author

In Situ Construction of Superhydrophobic Photothermal Coatings Based on Metal-Polyphenol Coordination Complex for Anti-/De-Icing Applications.

Polymers·2026
Same author

Segment-specific versus global cervical paraspinal muscle degeneration predicts sagittal balance and functional recovery after posterior surgery.

BMC musculoskeletal disorders·2026
Same author

Deep eutectic solvent extraction and AB-8 resin adsorption for rambutan shell polyphenol: Profiling and bioactivity assessments.

Food chemistry: X·2026
Same author

Transcriptomic and metabolomic insight into regulation of Cardiocrinum giganteum seed dormancy release induced by variable temperature stratification.

Planta·2026
Same author

Subparticle Operando Imaging for Probing Electrocatalytic Intermediates and Cation Effects.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Jul 21, 2025

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

483

Single-cell multimodal imaging uncovers energy conversion pathways in biohybrids.

Bing Fu1,2, Xianwen Mao1,3, Youngchan Park1

  • 1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, USA.

Nature Chemistry
|July 27, 2023
PubMed
Summary

Researchers developed a new microscopy technique to study microbe-semiconductor biohybrids. This method reveals how Ralstonia eutropha bacteria efficiently use electrons for bioplastic production, optimizing solar-to-chemical conversion.

More Related Videos

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
04:07

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments

Published on: February 23, 2024

1.3K
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.0K

Related Experiment Videos

Last Updated: Jul 21, 2025

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

483
Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
04:07

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments

Published on: February 23, 2024

1.3K
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
08:33

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

7.0K

Area of Science:

  • Biohybrid systems
  • Photocatalysis
  • Microbial electrosynthesis

Background:

  • Microbe-semiconductor biohybrids offer potential for solar-to-chemical conversion.
  • Understanding electron transport at the nano-bio interface is crucial for efficiency.
  • Heterogeneity in biohybrids complicates bulk measurement analysis.

Purpose of the Study:

  • To develop a quantitative multimodal microscopy platform for probing biohybrids.
  • To investigate electron transport mechanisms in Ralstonia eutropha.
  • To differentiate the roles of hydrogenases in bioplastic formation.

Main Methods:

  • Quantitative multimodal microscopy combining optical imaging and photocurrent mapping.
  • Single- to sub-cell/particle level analysis of biohybrids.
  • Investigating lithoautotrophic bacterium Ralstonia eutropha.

Main Results:

  • Uncovered critical roles of different hydrogenases in R. eutropha for bioplastic formation.
  • Discovered significant nanoampere-level electron uptake capability in R. eutropha.
  • Dissected cross-membrane electron transport pathways in the bacterium.

Conclusions:

  • The developed imaging platform enables detailed study of electron transport in biohybrids.
  • R. eutropha exhibits high electron uptake capacity, useful for chemical production.
  • This approach can guide engineering of R. eutropha for enhanced photocatalytic applications.