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ColE1 plasmid mobility: essential and conditional functions.

G J Warren, M W Saul, D J Sherratt

    Molecular & General Genetics : MGG
    |February 16, 1979
    PubMed
    Summary
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    Essential ColE1 plasmid transfer sequences were identified. Some ColE1 regions are not required for conjugal transfer, while others are crucial for mobilization by specific plasmids like R751 and R388.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • The conjugal transfer of ColE1 plasmid involves cis-acting sites and trans-acting products.
    • Understanding these elements is key to plasmid biology and genetic engineering.

    Purpose of the Study:

    • To delineate the specific DNA sequences essential for ColE1 plasmid conjugal transfer.
    • To investigate the requirements for ColE1 mobilization by different conjugative plasmids.

    Main Methods:

    • Cloning of ColE1 essential sequences into a non-transmissible plasmid vector to create a chimera.
    • Testing the transmissibility of the chimera using various conjugative plasmids (F'lac,pro, R64drd11, R751, R388).
    • Screening of ColE1 insertion mutants to identify regions involved in mobilization by R751 and R388.

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    Main Results:

    • A chimeric plasmid containing essential cis-acting and trans-acting sites was transmissible by incFI and incIalpha plasmids, but lacked colicin E1, immunity, and incompatibility genes.
    • This chimera showed deficient transfer mediated by incP (R751) and incW (R388) plasmids.
    • Three additional ColE1 regions, not previously identified as essential for transfer, were found necessary for mobilization by R751 and R388, with one overlapping the colicin E1 gene.

    Conclusions:

    • Colicin E1, immunity, and incompatibility genes are not essential for ColE1 conjugal transfer.
    • Specific ColE1 regions beyond the core transfer elements are required for mobilization by certain plasmid incompatibility groups.
    • These findings refine our understanding of plasmid conjugation mechanisms and host-plasmid interactions.