Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping the TAR vRNA Interaction with HIV-1 Integrase.

Viruses·2026
Same author

Unveiling the Structural Modifications of Cyanines to Target G‑Quadruplex DNA through Biophysical, Computational, and Transcriptome Analyses.

ACS omega·2026
Same author

Synthesis of BSA-Coated Iron Oxide Nanoparticles with Size Control for High-Performance <i>T</i><sub>1</sub> Contrast Agents in Magnetic Resonance Imaging.

Biomolecules·2026
Same author

In Search of the Most Significant Potential G-Quadruplexes in SARS-CoV-2 RNA: Genomic Analysis.

Viruses·2026
Same author

Ionic liquid-coated gold core polymeric nanoparticles for selective neutrophil hitchhiking towards endometriosis treatment.

Communications chemistry·2026
Same author

Hydrogen and Halogen Bond Interactions with 2,6-Dimethoxypyridine.

The journal of physical chemistry. A·2026

Related Experiment Video

Updated: Jul 21, 2025

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

10.6K

Selective Near-Infrared Blood Detection Driven by Ionic Liquid-Dye-Albumin Nanointeractions.

Donovan S Darlington1, Allison N Mahurin1, Karina Kapusta2

  • 1Department of Chemistry and Biochemistry, The University of Mississippi, University, Mississippi 38677, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|July 28, 2023
PubMed
Summary

Ionic liquids enhance near-infrared squaraine dye performance as a biosensor for human serum albumin (HSA) in blood. This "switch-on" fluorescence system improves detection by diversifying protein binding sites.

More Related Videos

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

Published on: May 3, 2011

14.7K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.9K

Related Experiment Videos

Last Updated: Jul 21, 2025

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

10.6K
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

Published on: May 3, 2011

14.7K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.9K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Serum albumin is abundant in blood, making it a target for biosensing.
  • Near-infrared (NIR) organic dyes are effective for albumin detection but struggle in whole blood.
  • Developing robust biosensors for albumin in complex biological fluids is crucial.

Purpose of the Study:

  • To enhance the performance of an NIR squaraine dye (SO3SQ) as a biosensor for albumin in blood.
  • To investigate the role of ionic liquids (ILs) in improving dye-based albumin detection.
  • To elucidate the mechanism behind the fluorescence enhancement in the presence of ILs and blood.

Main Methods:

  • Synthesis and characterization of the NIR squaraine dye SO3SQ.
  • Addition of biocompatible ionic liquids, specifically choline glycolate (1:1), to the dye system.
  • Fluorescence spectroscopy to measure dye emission in the presence of blood and albumin.
  • Dynamic Light Scattering (DLS), Isothermal Titration Calorimetry (ITC), and molecular dynamics simulations to study interactions.
  • Circular Dichroism (CD) spectroscopy to assess structural integrity of albumin and DNA.

Main Results:

  • The ionic liquid choline glycolate (1:1) at 160 mM significantly enhanced the fluorescence emission of SO3SQ in blood (a "switch-on" effect).
  • Fluorescence measurements indicated an impact on the tryptophan residue fluorescence of human serum albumin (HSA).
  • Apparent association constants for the dye-IL-albumin interaction were determined.
  • CD spectroscopy confirmed that the dye-IL system did not alter the secondary structures of albumin or DNA.

Conclusions:

  • The addition of specific ionic liquids, like choline glycolate, can overcome the limitations of NIR dyes in whole blood for albumin sensing.
  • The enhanced fluorescence is attributed to the diversification of binding sites within albumin, mediated by the IL-dye-albumin complex.
  • This IL-enhanced dye system shows promise for improved biosensing applications in complex biological samples.