Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel antimicrobial peptide Spamprin<sub>4-23</sub> from Scylla paramamosain exhibiting potent antimicrobial activity and protective effects against Vibrio alginolyticus.

Fish & shellfish immunology·2026
Same author

TimeX: Phenotype Onset Extraction from Clinical Narratives.

npj health systems·2026
Same author

Harnessing Patient-Generated Data for Rare Disease Knowledge Enrichment: A Pilot Study.

Studies in health technology and informatics·2026
Same author

Interpretable fine-tuned large language models facilitate making genetic test decisions for rare diseases.

NPJ digital medicine·2026
Same author

RMScratcher: An edge-AI platform for high-throughput analysis of rodent scratching behavior.

Journal of neuroscience methods·2026
Same author

Marine antimicrobial peptide scyreprocin exhibits therapeutic potential against multidrug-resistant bacteria in wound and lung infections with low resistance.

International journal of biological macromolecules·2026

Related Experiment Video

Updated: Jul 21, 2025

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

2.9K

Analysis of intercellular communication in the osteosarcoma microenvironment based on single cell sequencing data.

Fangyi Chen1, Jiao Liu2, Ting Yang3

  • 1Department of Orthopedics, Jinshan Hospital, Fudan University, Shanghai, China.

Journal of Bone Oncology
|July 28, 2023
PubMed
Summary

Single-cell analysis reveals diverse cell types and communication networks within osteosarcoma tumors. These findings illuminate the tumor microenvironment

Keywords:
Intercellular communicationMicroenvironmentOsteosarcomaSingle-cell sequencing

More Related Videos

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.3K
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.3K

Related Experiment Videos

Last Updated: Jul 21, 2025

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

2.9K
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.3K
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.3K

Area of Science:

  • Oncology
  • Genomics
  • Immunology

Background:

  • Osteosarcoma (OS) is a prevalent bone cancer in young individuals with stagnant survival rates.
  • Understanding the tumor microenvironment is crucial for developing novel therapeutic strategies.

Purpose of the Study:

  • To comprehensively analyze the cellular composition and intercellular communication within the osteosarcoma tumor microenvironment.
  • To identify potential therapeutic targets by examining cell-cell interactions and their association with patient survival.

Main Methods:

  • Single-cell RNA sequencing was performed on tumor samples from six untreated osteosarcoma patients.
  • Unbiased clustering and canonical markers identified nine major cell types.
  • Copy number variation (CNV) analysis and RNA velocity trajectory analysis were employed.

Main Results:

  • Significant heterogeneity in cellular composition was observed across osteosarcoma samples.
  • Myeloid cells were the most abundant cell type, followed by osteoblastic cells and tumor-infiltrating lymphocytes (TILs).
  • Intercellular communication pathways, particularly between myeloid and osteoblastic cells, were identified, with 21 ligand-receptor pairs linked to survival outcomes.

Conclusions:

  • The study provides a detailed map of the osteosarcoma cellular landscape and communication networks.
  • Chemotherapy appears to influence cellular communication within the tumor microenvironment.
  • These findings offer insights into OS progression and potential targets for future treatments.