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Updated: Jul 21, 2025

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
Published on: March 28, 2025
Mycobacterium tuberculosis Rv0494 Protein Contributes to Mycobacterial Persistence
Lei Ji1, Tingting Jiang1, Xin Zhao2
1School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, People's Republic of China.
Purpose:
Fatty acid metabolism plays an important role in the survival and pathogenesis of Mycobacterium tuberculosis. During dormancy, lipids are considered to be the main source of energy. A previous study found that Rv0494 is a starvation-inducible, lipid-responsive transcriptional regulator. However, the role of Rv0494 in bacterial persister survival has not been studied.
Methods:
We constructed a Rv0494 deletion mutant strain of Mycobacterium tuberculosis H37Rv and evaluated the susceptibility of the mutant strain to antibiotics using a persistence test.
Results:
We found that mutations in Rv0494 lead to survival defects of persisters, which reflected in increased sensitivity to isoniazid.
Conclusion:
We conclude that Rv0494 is important for persister survival and may serve as a good target for developing new antibiotics that kill persister bacteria for improved treatment of persistent bacterial infections.
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