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Updated: Jul 21, 2025

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
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Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET

Jae Yang1,2, Veronika Vrbovská3, Tilman Franke3

  • 1Department of Biochemistry, University of Wisconsin, Madison, WI USA.

Biorxiv : the Preprint Server for Biology
|July 28, 2023
PubMed
Summary

We developed an integrated fluorescence light microscope (iFLM) for cryo-FIB-SEM, enabling precise 3D correlative workflows. This automated method efficiently prepares cryo-preserved cellular lamellae for cryo-electron tomography (cryo-ET) analysis.

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