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Related Concept Videos

Replicative Cell Senescence02:15

Replicative Cell Senescence

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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
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Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
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Aging01:26

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Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
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Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
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Updated: Jul 21, 2025

Techniques to Induce and Quantify Cellular Senescence
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Techniques to Induce and Quantify Cellular Senescence

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LEF1 isoforms regulate cellular senescence and aging.

Minxue Jia1,2, Khaled Sayed1,3, Maria G Kapetanaki1

  • 1Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Biorxiv : the Preprint Server for Biology
|July 28, 2023
PubMed
Summary
This summary is machine-generated.

Lymphoid Enhancer Binding Factor 1 (LEF1) is a key regulator of aging and cellular senescence. Its differential expression is linked to age-related lung diseases like Idiopathic Pulmonary Fibrosis (IPF).

Keywords:
Cellular SenescenceIPFLEF1Lung

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Area of Science:

  • Immunology
  • Molecular Biology
  • Gerontology

Background:

  • Cellular senescence is a key mechanism in aging and age-related diseases.
  • High-throughput data and analysis algorithms aid in identifying disease-driving pathways.
  • Understanding aging mechanisms is crucial for preventing and treating age-related pathologies.

Approach:

  • Comparative transcriptomic analysis of aged versus young human and mouse lung immune cells (macrophages, T cells, B cells).
  • Validation of findings in cell culture and human lung tissue.
  • Investigated the role of Lymphoid Enhancer Binding Factor 1 (LEF1) in aging and senescence.

Key Points:

  • Lymphoid Enhancer Binding Factor 1 (LEF1) identified as a crucial age-associated gene regulator across multiple cell types and species.
  • Differential expression of LEF1 isoforms is a critical mechanism in cellular senescence.
  • LEF1 dysregulation observed in lung tissue from Idiopathic Pulmonary Fibrosis (IPF) patients.

Conclusions:

  • LEF1 is a significant factor in the aging process.
  • Differential regulation of LEF1 is associated with cellular senescence in both humans and mice.
  • Findings highlight LEF1 as a potential therapeutic target for age-related diseases.