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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
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A functional microbiome catalog crowdsourced from North American rivers.
Biorxiv : the Preprint Server for Biology
|July 28, 2023
Summary
A community science effort created the Genome Resolved Open Watersheds database (GROWdb), profiling thousands of microbial genomes across U.S. rivers. This resource reveals drivers of river microbiome structure and function, enabling predictive watershed modeling.
Area of Science:
- Environmental microbiology
- Riverine biogeochemistry
- Metagenomics and bioinformatics
Background:
- Understanding river microbiomes is crucial for predicting elemental cycles and water quality under anthropogenic stress.
- Current knowledge is limited by a lack of genome-resolved functional data and broad geographic sampling.
Conclusions:
- River microbiome structure and function are predictable, extending the River Continuum Concept with microbial functional traits.
- GROWdb provides a foundational resource for interdisciplinary research in watershed science.
- The database supports the development of microbiome-based management practices for improved water quality.
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