Vitamin A transport in plasma of diabetic patients

The effects of diabetes on the vitamin A metabolism were studied. The concentrations of plasma retinol and retinyl ester were measured in diabetic patients using high-performance liquid chromatography (HPLC). In diabetic patients, the mean level of retinyl ester was significantly elevated compared to that of normal subjects (diabetes, 68.3 +/- 42.5 IU/100 ml of plasma; study I, 87.6 +/- 64.3 IU/100 ml of plasma; study II vs. normal, 29.8 +/- 10.3 IU/100 ml of plasma). The ultracentrifugal and column chromatographic studies were carried out to examine the distribution of retinyl ester in patient's plasma. Ultracentrifugation study showed that the mean of 72.6% of retinyl ester was recovered in the lipoproteins of density higher than 1.006 and the remainder was recovered in the chylomicron (d less than 1.006). Gel filtration profiles for the separation of plasma vitamin A demonstrated that retinyl ester presented in the HDL, LDL and larger molecular size lipoprotein (suspected to be VLDL) as well as in the chylomicron.