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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
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PIWIL1 interacting RNA piR-017724 inhibits proliferation, invasion, and migration, and inhibits the development of

Yi-Jing Wu1, Jie Wang1, Peng Zhang2

  • 1Medical School of Nantong University, Nantong Third People's Hospital, Affiliated Nantong Hospital 3 of Nantong University, Nantong, China.

Frontiers in Oncology
|July 28, 2023
PubMed
Abstract

Insights

PIWI-interacting RNA (piRNA) piR-017724 is downregulated in hepatocellular carcinoma (HCC), inhibiting cancer cell proliferation, migration, and invasion. This piRNA may impact HCC development via the protein PLIN3, offering potential therapeutic insights.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) is a major cause of cancer death globally.
  • PIWI-interacting RNAs (piRNAs) are implicated in various cancers, but their role in HCC is not well understood.
  • Limited studies exist on piRNA mechanisms in HCC development and progression.

Purpose of the Study:

  • To investigate the expression and function of piR-017724 in hepatocellular carcinoma.
  • To determine the clinical and prognostic significance of piR-017724 in HCC patients.
  • To elucidate the downstream target and mechanism of piR-017724 in HCC progression.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to detect piR-017724 expression in HCC tissues and cells.
  • Analysis of clinical data to assess the prognostic value of piR-017724.
  • In vitro experiments involving targeted silencing and overexpression of piR-017724 in HCC cells.
  • High-throughput sequencing and database analysis to identify downstream targets.

Main Results:

  • piR-017724 was significantly downregulated in HCC tissues and cells, correlating with advanced tumor stage and poorer prognosis.
  • Inhibition of piR-017724 promoted HCC cell proliferation, migration, and invasion, while its overexpression had the opposite effect.
  • piR-017724 did not significantly affect HCC cell apoptosis.
  • A reciprocal relationship between piR-017724 and PLIN3 was confirmed.

Conclusions:

  • Lowly expressed piR-017724 acts as a tumor suppressor in HCC by inhibiting proliferation, migration, and invasion.
  • The tumor-suppressive function of piR-017724 may be mediated through its downstream target PLIN3.
  • piR-017724 presents a potential biomarker and therapeutic target for hepatocellular carcinoma.
Keywords:
PLIN3hepatocellular carcinomainvasionmigrationpiR-017724proliferation

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