Engineering hetero-structural iron nanozyme decorated liposome with a self-cascade catalysis performance

Metal-based enzyme mimics are considered as acceptable agents in fabricating heterogeneous biocomposites through valency integrations because of their biomedical or biological properties. As the basic substitute, it delights us to utilize Fe₃O₄ nanoparticles (NPs) as metallic enzymes and overcome the limitation of peroxide-like enzymatic activity in physiological conditions. In this work, we present the fabrication of a soy phosphatidylcholine/Fe₃O₄@Ag/GOx (SFAG) biocomposite as a cascade enzyme, which exhibits a peroxidase-like property in kinetic processes, as shown from an analysis of the glucose detection processes. We also explored the mechanism of an ultrasound & microfluidic approach for the synthesis of SFAG. The resultant SFAG implies a characteristic absorption peak (652 nm), size (55 μm), and surface charge (-32.93 ± 2.58 mV). This is utilized to confirm the peroxidase-like activity by catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ under physiological conditions. But also, SFAG conveys a positive effect on the peroxidase-like activity at pH = 5.8, 7.4, and 8.0. The Michaelis-Menten parameters (K_m) and the V_max values of H₂O₂ are 1.914 mM and 1.429 × 10^-7 M s^-1, which further confirms the catalytic performances of the SFAG structure. The established platform was also used successfully for the determination of glucose in PBS and diluted synthetic blood with excellent sensitivity and stability. The relative selection and sensitivity show that the SFAG structure has a great possibility as a cascade metallic enzyme in chemokinetic works.