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Updated: Jul 21, 2025
Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
Anna S Efimova1,2, Mariya A Ustimova1, Nelly S Chmelyuk3
1Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119334 Moscow, Russia.
Abstract:
New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influenced their binding modes, binding affinities, and spectroscopic responses. Biological experiments showed the low toxicity of the obtained dyes and their applicability as selective dyes for mitochondria in living cells.
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